期刊
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
卷 117, 期 4, 页码 523-531出版社
WILEY
DOI: 10.1002/ejlt.201400327
关键词
Fatty acid desaturase; Mesocarp; O; L ratio; Olive (Olea europaea L; ); Seed
资金
- National Institute of Genetic Engineering and Biotechnology of Iran [437]
To understand the molecular basis of the higher oleic/linoleic acid content in Mari and Koroneiki with respect to Shengeh and Arbequina olive cultivars, we have focused on analysis of fatty acids, as well as expression levels of OeSAD and three oleate desaturase genes during fruit development and ripening. The mesocarp oil content was determined using a Minispec NMR. Moreover, oleic and linoleic acid contents were recorded by GC. Quantitative real time PCR was used for gene expression analysis. Mari and Shengeh exhibited the highest and lowest levels of OeSAD gene expression, respectively, which corresponded fully to a change in the pattern of oleic acid and oil accumulation throughout mesocarp development. Regarding oleate desaturases, a decreasing pattern of OeFAD2-1 and OeFAD6 transcripts accompanied by an increasing trend of OeFAD2-2 expression during mesocarp development showed that, coinciding with oil accumulation, linoleic acid content is mainly determined by the OeFAD2-2 gene. In conclusion, the higher oleic/linoleic ratio in Mari and Koroneiki cultivars compared to Shengeh and Arbequina was due to much higher expression levels of OeSAD and lower expression levels of OeFAD2-2. Practical applications: Increasing the oleic to linoleic acid ratio has become one of the major goals for researchers in the area of plant lipid modification. To this end, understanding the mechanisms by which fatty acid composition is determined during olive fruit development and ripening would represent an important step toward the ultimate goal of regulating these processes in a directed and predictable manner.
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