A review of lipidomic technologies applicable to sphingolipidomics and their relevant applications

Sphingolipiclomics, a branch of lipidomics, focuses on the large-scale study of the cellular sphingolipidomes. In the current review, two main approaches for the analysis of cellular sphingolipidomes (i.e. LC-MS- or LC-MS/MS-based approach and shotgun lipidomics-based approach) are briefly discussed. Their advantages, some considerations of these methods, and recent applications Of these approaches are summarized. It is the authors' sincere hope that this review article will add to the readers understanding of the advantages and limitations of each developed method for die analysis of a cellular sphingolipidome.