期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 43, 期 6, 页码 1667-1675出版社
WILEY
DOI: 10.1002/eji.201343312
关键词
Bone marrow; Patrolling monocytes; Sphingosine-1 phosphate; Survival; Trafficking
类别
资金
- FINOVI foundation
- Agence Nationale de la Recherche (ANR JC sphinks)
- European Research council [ERC-Stg 281025]
- Institut National de la Sante et de la Recherche Medicale (INSERM)
Patrolling Ly6C(-) monocytes are blood-circulating cells that play a role in inflammation and in the defense against pathogens. Here, we show that similar to natural killer (NK) cells, patrolling monocytes express high levels of S1PR5, a G-coupled receptor for sphingosine-1 phosphate. We found that S1pr5(-/-) mice lack peripheral Ly6C(-) monocytes but have a normal number of these cells in the bone marrow (BM). Various lines of evidence exclude a direct contribution of S1PR5 in the survival of Ly6C(-) monocytes at the periphery. Rather, our data support a role for S1PR5 in the egress of Ly6C(-) monocytes from the BM. In particular, we observed a reduced frequency of patrolling monocytes in BM sinusoids of S1PR5 KO mice. Unexpectedly, S1P was not a chemoattractant for patrolling monocytes and had no significant effect on their viability in vitro. Moreover, the disruption of S1P gradients in vivo did not alter Ly6C(-) monocyte trafficking and viability. These data suggest that S1PR5 regulates the trafficking of monocytes via a mechanism independent of S1P gradients.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据