期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 43, 期 11, 页码 2832-2844出版社
WILEY
DOI: 10.1002/eji.201343600
关键词
Antigen processing; Carbon monoxide; Cross-presentation; Endocytosis; Heme oxygenase-1
类别
资金
- La Region Pays de la Loire through Chaire d'excellence program
- IMBIO program
- l'Agence de la Biomedecine
- Ministere de la Recherche
- Fondation CENTAURE
- Fondation Progreffe
- La Region Pays De La Loire
- INSERM CDD grant
- ECOS France-Chile grant
- Millennium Institute on Immunology and Immunotherapy from Chile [P09/016-F]
Heme oxygenase-1 (HO-1) inhibits immune responses and inflammatory reactions via the catabolism of heme into carbon monoxide (CO), Fe2+, and biliverdin. We have previously shown that either induction of HO-1 or treatment with exogenous CO inhibits LPS-induced maturation of dendritic cells (DCs) and protects in vivo and in vitro antigen-specific inflammation. Here, we evaluated the capacity of HO-1 and CO to regulate antigen presentation on MHC class I and MHC class II molecules by LPS-treated DCs. We observed that HO-1 and CO treatment significantly inhibited the capacity of DCs to present soluble antigens to T cells. Inhibition was restricted to soluble OVA protein, as no inhibition was observed for antigenic OVA-derived peptides, bead-bound OVA protein, or OVA as an endogenous antigen. Inhibition of soluble antigen presentation was not due to reduced antigen uptake by DCs, as endocytosis remained functional after HO-1 induction and CO treatment. On the contrary, CO significantly reduced the efficiency of fusion between late endosomes and lysosomes and not by phagosomes and lysosomes. These data suggest that HO-1 and CO can inhibit the ability of LPS-treated DCs to present exogenous soluble antigens to naive T cells by blocking antigen trafficking at the level of late endosome-lysosome fusion.
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