期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 42, 期 1, 页码 165-175出版社
WILEY
DOI: 10.1002/eji.201141833
关键词
Allergy; BCG; DC subsets; Hygiene hypothesis; Treg cell
类别
资金
- Manitoba Health Research Council/Manitoba Institute of Children Health
- Canadian Institutes of Health Research (CIHR)
The hygiene hypothesis has suggested an inhibitory effect of infections on allergic diseases, but the related mechanism remains unclear. We recently reported that DCs played a critical role in Mycobacterium bovis Bacille CalmetteGuerin (BCG)-mediated inhibition of allergy, which depended on IL-12 and IL-10-related mechanisms. Here, we tested the hypothesis that BCG infection could modulate the function of DC subsets, which might in turn inhibit allergic responses through different mechanisms. We sorted CD8a+ and CD8a- DCs from BCG-infected mice and tested their ability to modulate Th2-cell responses to ovalbumin (OVA) using in vitro and in vivo approaches. We found that both DC subsets could inhibit the allergic Th2-cell response in both a DC:T-cell co-culture system and after adoptive transfer. These subsets exhibited different co-stimulatory marker expression and cytokine production patterns and were different in inducing Th1 and Treg cells. Specifically, we found that CD8a+ DCs produced higher IL-12, inducing higher Th1 cell response, while CD8a- DCs expressed higher ICOS-L and produced higher IL-10, inducing CD4+CD25+FoxP3+Treg cells with IL-10 production and membrane-bound TGF-beta expression. The finding suggests that one infection may inhibit allergy by both immune deviation and regulation mechanisms through modulation of DC subsets.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据