期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 40, 期 4, 页码 1127-1138出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/eji.200939664
关键词
Apoptosis; Cyclin-dependent kinase inhibition; Inflammation; Neutrophil
类别
资金
- MRC [G0601481]
- Wellcome Trust [WT082181]
- Medical Research Council [G0601481, G9900991B] Funding Source: researchfish
- MRC [G0601481] Funding Source: UKRI
Successful resolution of inflammation requires inflammatory cells such as neutrophils to undergo apoptosis prior to non-inflammatory phagocytosis by professional phagocytes. Recently, cyclin-dependent kinase (CDK) inhibitors (e.g. R-roscovitine) have been shown to induce neutrophil apoptosis and enhance the resolution of inflammation. Interestingly, NF-kappa B and MAPK pathways and key endogenous survival proteins (typified by Mcl-1) are involved in the regulation of neutrophil apoptosis and, in cancer-cell lines, have been implicated as possible targets of CDK inhibitors. Here, we demonstrate that R-roscovitine over-rides TNF-alpha and LPS-induced survival (determined by morphological examination and binding of fluorescently labelled annexin-V) of isolated peripheral blood neutrophils. This effect did not appear to be mediated via effects on early markers of neutrophil activation (e.g. surface marker expression, shape change, aggregation and superoxide anion generation), by direct inhibition of NF-kappa B activation (assessed by cytoplasmic I kappa B alpha proteolysis and NF-kappa B p65 subunit translocation) and ERK activation (determined by specific ERK phosphorylation) but due to down-regulation (at protein and mRNA level) of the survival protein Mcl-1 but not the pro-apoptotic bcl-2 homologue Bim. These findings suggest that key endogenous survival proteins may be the targets of CDK inhibitors and consequently may be of critical importance in the resolution of inflammation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据