期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 39, 期 6, 页码 1587-1596出版社
WILEY
DOI: 10.1002/eji.200838907
关键词
Chemokine; Epithelium; Flagellin; Lung inflammation; TLR5
类别
资金
- CNRS
- French Ministry of Education t
- European Community [INCO-CT-2006-032296]
- Region Nord Pas de Calais
- UK Medical Research Council
- Medical Research Council [G0400795] Funding Source: researchfish
- MRC [G0400795] Funding Source: UKRI
Bacterial products (such as endotoxins and flagellin) trigger innate immune responses through TLRs. Flagellin-induced signalling involves TLR5 and MyD88 and, according to some reports, TLR4. Whereas epithelial and dendritic cells are stimulated by flagellin in vitro, the cell contribution to the in vivo response is still unclear. Here, we studied the respective roles of radioresistant and radiosensitive cells in flagellin-induced airway inflammation in mice. We found that i.n. delivery of flagellin elicits a transient change in respiratory function and an acute, pro-inflammatory response in the lungs, characterized by TLR5- and MyD88-dependent chemokine secretion and neutrophil recruitment. In contrast, TLR4, CD14 and TRIF were not essential for flagellin-mediated responses, indicating that TLR4 does not cooperate with TLR5 in the lungs. Respiratory function, chemokine secretion and airway infiltration by neutrophils were dependent on radioresistant, TLR5-expressing cells. Furthermore, lung haematopoietic cells also responded to flagellin by activating TNF-alpha production. We suggest that the radioresistant lung epithelial cells are essential for initiating early, TLR5-dependent signalling in response to flagellin and thus triggering the lung's innate immune responses.
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