期刊
EUROPEAN JOURNAL OF ENTOMOLOGY
卷 107, 期 2, 页码 147-156出版社
CZECH ACAD SCI, INST ENTOMOLOGY
DOI: 10.14411/eje.2010.020
关键词
Adelgidae; aphids; species identification; systematics; DNA barcoding
类别
资金
- Grant Agency of the Academy of Sciences of the Czech Republic (Prague) [A600960705]
- Institute of Entomology [Z50070508]
- Grant Agency of the Czech Republic, Prague [521/08/H042]
The sequence diversity in the mitochondrial cytochrome-c oxidase I (COI) gene was evaluated as a tool for resolving differences among species of European adelgids collected from several localities across the Czech Republic. Members of 7 genera and 16 species were examined, and as outgroups, two species of Phylloxeridae were used. Sequence divergences within species were on average less than 0.15%, whereas divergences between species ranged from 0.0 to 4.12% for congeneric and to 13.24% for intergeneric comparisons. It is concluded that DNA barcoding of Adelgidae is a powerful tool for identifying genera, but at the species level it works only in those cases where there are no species complexes. Nevertheless, it can be used as a complement to traditional, morphological taxonomy.
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