期刊
EUROPEAN JOURNAL OF CELL BIOLOGY
卷 93, 期 4, 页码 157-169出版社
ELSEVIER GMBH
DOI: 10.1016/j.ejcb.2014.03.002
关键词
Cancer cell extravasation; Invadopodium; Invadosome; Invasion; Plectin; Vimentin
类别
资金
- Japanese Society for the Promotion of Science [22570131, B22390301, 24659708]
- Austrian Science Research Fund [1413-B09]
- Austrian Science Fund (FWF) [I 1207, P 23729] Funding Source: researchfish
- Grants-in-Aid for Scientific Research [22570131] Funding Source: KAKEN
To investigate the molecular mechanisms of cancer metastasis, we have isolated a high-metastatic bladder cancer cell subpopulation from a low-metastatic cell line by using an in vivo selection system. Cells in the subpopulation showed a high ability to form invadopodia, the filamentous actin (F-actin)-based membrane protrusions that play an essential role in cancer cell invasion. Analysis of the gene expression profile revealed that the expression of an intermediate filament (IF) protein, vimentin and a cytoskeletal linker protein, plectin was up-regulated in the high-metastatic subpopulation compared with the low metastatic cell line. Here we report a novel role of vimentin IF and plectin in metastasis. In invasive bladder cancer cells, the vimentin IF-plectin-invadopodia F-actin link was formed. Disruption of this link severely impaired invadopodia formation, reducing the capacities of extracellular matrix degradation, transendothelial migration and metastasis. In addition, the vimentin assembly into the filaments was required for invadopodia formation. Our results suggest that plectin anchoring invadopodia to vimentin IF scaffolds and stabilizes invadopodia, which is a critical molecular process for cancer cell invasion and extravasation for metastasis. (C) 2014 Elsevier GmbH. All rights reserved.
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