期刊
EUROPEAN JOURNAL OF CELL BIOLOGY
卷 93, 期 10-12, 页码 413-423出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.ejcb.2014.07.008
关键词
Invadopodia; Actin cytoskeleton; Metastasis; Matrix degradation; 3D invasion; Epithelial-mesenchymal transition
类别
资金
- Spanish Ministry of Science and Innovation [BFU2010-21374/BMC]
- CIBERNED (Instituto de Salud Carlos III)
- Medical Research Council [G1100041]
- European Union [237946]
- Cancer Research UK
- Comunidad Autonoma de Madrid (CAM)
- EMBO
- CSIC
- Cancer Research UK [15673] Funding Source: researchfish
- Medical Research Council [G1100041] Funding Source: researchfish
- Worldwide Cancer Research [11-0119] Funding Source: researchfish
- MRC [G1100041] Funding Source: UKRI
Actin filament assembly and reorganisation during cell migration and invasion into extracellular matrices is a well-documented phenomenon. Among actin-binding proteins regulating its polymerisation, the members of the WASP (Wiskott Aldrich Syndrome Protein) family are generally thought to play the most significant role in supporting cell invasiveness. In situ, cytosolic N-WASP (neural WASP) is associated with a partner protein termed WIP (WASP Interacting Protein) that is bound to the N-terminal domain of N-WASP. Despite much effort, rather little is known about the role of WIP in regulating N-WASP and consequent actin-filament assembly. Even less is known about the function of WIP within the specialised cell adhesion and attachment structures known as podosomes and invadopodia. In particular, whilst the interaction of WIP with known participants in the development and maturation of invadopodia such as N-WASP, the Arp2/3 complex and cortactin has been described, little is known concerning the direct contribution of WIP to invadopodia and its potential role as a regulator of cancer cell invasion. In this report, we use 2D and 3D culture systems to describe the role played by WIP in modulating the morphology and invasiveness of metastatic breast cancer cells in vitro, as well as its effect on the process of mesenchymal-epithelial transition (MET) seen in these cells. We demonstrate that WIP is necessary for invadopodium formation and matrix degradation by basal breast cancer cells, but not sufficient to induce invasiveness in luminal cells. Crown Copyright (C) 2014 Published by Elsevier GmbH. All rights reserved.
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