期刊
EUROPEAN JOURNAL OF CELL BIOLOGY
卷 89, 期 11, 页码 788-798出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.ejcb.2010.07.001
关键词
Galectin-3; Apical trafficking; Endosomes; COS-1; MDCK; Sorting
类别
资金
- Deutsche Forschungsgemeinschaft (DFG), Bonn, Germany [JA 1033, Graduiertenkolleg 1216, Sonderforschungsbereich 593]
In epithelial cells, the (beta-galactoside-binding lectin galectin-3 mediates the non-raft-dependent glycoprotein targeting to the apical membrane domain. In this study, we aimed to identify intracellular. compartments involved in the trafficking of galectin-3. By studying fluorescent fusion proteins in living cells, we could show that galectin-3 accumulates intracellularly in acidified endosomes. Total internal reflection fluorescence microscopy studies of the apical surface of polarized MDCK cells revealed that galectin-3 is enriched in tubular and vesicular Rab1 1-positive recycling endosomes in the vicinity of the apical cell surface. These endosomal organelles are candidate compartments for the association between galectin-3 and exocytic apical cargo. Crown Copyright (C) 2010 Published by Elsevier GmbH. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据