期刊
EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY
卷 108, 期 2, 页码 217-225出版社
SPRINGER
DOI: 10.1007/s00421-009-1201-8
关键词
ERK; Hsp72; Neutrophils; NF-kappa beta; Phagocytosis and fungicidal capacity; PI3K; TLR-2
资金
- PRI [2PR04A076, PRI06A172]
- MICINN-FEDER [DEP200910041]
- Consejeria de Educacion, Ciencia y Tecnologia of Junta de Extremadura
- Fondo Social Europeo
This study evaluated the role of toll like receptor 2 (TLR-2) in the interaction of 72 kDa extracellular heat shock protein (Hsp72, a stress-inducible protein) with neutrophils and the participation on TLR-2 in the stimulation of neutrophil phagocytic and fungicidal capacities by post-exercise physiological concentrations of Hsp72. Human peripheral blood neutrophils were incubated with fluorescein isothiocyanate-conjugated Hsp72, and were analyzed by immunofluorescence microscopy and flow cytometry. Both methods revealed an interaction of Hsp72 with neutrophils. In addition, when neutrophils were pre-incubated with an anti-TLR-2 antibody this interaction was clearly decreased. Post-exercise circulating concentration of Hsp72 (8.6 ng/ml) stimulated the phagocytic and fungicidal capacities of neutrophils and this effect could be also blocked using an antibody against TLR-2. Phosphatidylinositol-3-kinase (PI3K), extracellular signal-regulated kinase (ERK) and the nuclear transcription factor kappa beta (NF-kappa I-2) were found to be involved in the signaling process, confirming the participation of TLR-2 in the stimulation of neutrophil function by Hsp72. In conclusion, TLR-2 is involved at least in part, in the stimulation of neutrophil phagocytic and fungicidal capacities induced by post-exercise physiological concentrations of Hsp72.
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