Preparation of high-purity phosphatidylcholine from rapeseed oil gums by cation ion-exchange resin

High-purity phosphatidylcholine (PC) was widely used in the food and pharmaceutical. The purification of PC from rapeseed gums is the urgent need to be resolved. In this study, crude PC extraction from the pretreatment of rapeseed oil gums with ethanol was used as the raw material to prepare high-purity PC using ion-exchange resin. ZGD152 was selected from six cation ion-exchange resins to separate rapeseed PC. The adsorption characteristics of PC and phosphatidylethanolamine (PE) on ZGD152 resins have been compared. The equilibrium absorption data of PC and PE on ZGD152 resin were fitted to Langmuir and Freundlich model. The adsorption thermodynamic parameters were also evaluated on pseudo-first-order model. Experimental optimization of dynamic adsorption and desorption was performed in a glass column packed with ZGD152 resin. The PC was successfully separated with gradient elution using 20 % (4 BV), 60 % (13 BV) aqueous ethanol solutions, and 5 wt% hydrochloric acid (8 BV). The content of PC was increased from 50.2 to 87.7 % in the final product with the recovery of 58.2 %. The results indicated that the ZGD152 resin was an effective separation media for the purification of PC from the rapeseed phospholipids, with potential application in the future.