期刊
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
卷 234, 期 4, 页码 679-687出版社
SPRINGER
DOI: 10.1007/s00217-012-1678-y
关键词
GABA accumulation; Germinated soybean; Glutamate decarboxylase (GAD); Diamine oxidase activity (DAO); Hypoxia stress
资金
- Natural Science Foundation of China [31071581]
- fundamental research funds for the central universities [KYZ200917]
High levels of gamma-aminobutyric acid (GABA) accumulate in plant tissues under various stresses and exogenous additives. The purpose of this research is to provide an effective finding that can prove a rapid accumulation of GABA in germinated soybean (Glycine max L.) in response to different additives under hypoxia. Hypoxia-induced GABA accumulation in soybean embryo resulted in part from polyamine oxidation. Response to different concentration of glutamate (Glu), pyridoxal phosphate, arginine, CuCl2, NaCl, and CaCl2, a significant difference including GABA accumulation, changes of Glutamate decarboxylase (GAD), and Diamine oxidase activity (DAO) activity in germinated soybean under hypoxia occurred (p < 0.05) and the maximum accumulation of GABA were 4.07, 3.02, 3.50, 3.26, 4.00, and 3.30 g kg(-1) DW respectively, which were significantly higher than those germinated soybean under normal culture (CK) and hypoxia culture (CK0) (p < 0.05). The GAD and DAO have different distributions in cotyledon and embryo of germinated soybean, and the enzyme activity mainly located in embryo of germinated soybean. Germinated soybean is a good resource of GABA-rich food. Different additives have significant effects on GABA production, among which Glu and NaCl are ideal material for GABA accumulation.
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