期刊
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
卷 42, 期 1, 页码 33-46出版社
SPRINGER
DOI: 10.1007/s00249-012-0868-7
关键词
Aquaporin; NMR; Red blood cell; Water channel protein; Water permeability
类别
资金
- Ministry of Education and Research of The Iuliu Hatieganu'' University of Medicine and Pharmacy Cluj-Napoca
- Academy of Medical Sciences
- National Council for Science and Technology
- National Council for Higher University Scientific Research (Romania)
- Taronga Zoo
- University of Sydney (Australia)
- Wellcome Trust (UK)
- National Science Foundation (USA)
NMR measurements of the diffusional permeability of the human adult red blood cell (RBC) membrane to water (P (d)) and of the activation energy (E (a,d)) of the process furnished values of P (d) similar to 4 x 10(-3) cm/s at 25 A degrees C and similar to 6.1 x 10(-3) cm/s at 37 A degrees C, and E (a,d) similar to 26 kJ/mol. Comparative NMR measurements for other species showed: (1) monotremes (echidna and platypus), chicken, little penguin, and saltwater crocodile have the lowest P (d) values; (2) sheep, cow, and elephant have P (d) values lower than human P (d) values; (3) cat, horse, alpaca, and camel have P (d) values close to those of humans; (4) guinea pig, dog, dingo, agile wallaby, red-necked wallaby, Eastern grey kangaroo, and red kangaroo have P (d) values higher than those of humans; (5) mouse, rat, rabbit, and small and medium size marsupials have the highest values of P (d) (> 8.0 x 10(-3) cm/s at 25 A degrees C and > 10.0 x 10(-3) cm/s at 37 A degrees C). There are peculiarities of E (a,d) values for the RBCs from different species. The maximum inhibition of diffusional permeability of RBCs induced by incubation with p-chloromercuribenzene sulfonate varied between 0 % (for the chicken and little penguin) to similar to 50 % (for human, mouse, cat, sheep, horse, camel, and Indian elephant), and similar to 60-75 % (for rat, guinea pig, rabbit, dog, alpaca, and all marsupials). These results indicate that no water channel proteins (WCPs) or aquaporins are present in the membrane of RBCs from monotremes (echidna, platypus), chicken, little penguin and saltwater crocodile whereas WCPs from the membranes of RBCs from marsupials have peculiarities.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据