期刊
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
卷 38, 期 8, 页码 1049-1059出版社
SPRINGER
DOI: 10.1007/s00249-009-0507-0
关键词
E. coli; Cell suspension; Dielectric spectroscopy; Impedance analysis; Membrane potential; Voltage-sensitive dyes
类别
资金
- National Science Foundation [0547427]
- Direct For Education and Human Resources
- Division Of Human Resource Development [0547427] Funding Source: National Science Foundation
In this paper we demonstrate a quantitative way to measure the membrane potential of live cells by dielectric spectroscopy. We also show that the values of the membrane potential obtained using our technique are in good agreement with those obtained using traditional methods-voltage-sensitive dyes. The membrane potential is determined by fitting the experimental dielectric dispersion curves with the dispersion curves obtained from a theoretical model. Variations in the membrane potential were induced by modifying the concentration of potassium chloride in the solution of the cell suspension in the presence of valinomycin. For exemplification of the method, E. coli was chosen for our experiments.
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