期刊
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
卷 39, 期 2, 页码 337-343出版社
SPRINGER
DOI: 10.1007/s00249-009-0502-5
关键词
Biophysics; Single molecule; Magnetic tweezers; Lambda exonuclease; Fragile X syndrome
类别
资金
- National Research Council
- NINDS, NIH
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [Z01NS003047, ZIANS003047] Funding Source: NIH RePORTER
Fragile X syndrome and other trinucleotide diseases are characterized by an elongation of a repeating DNA triplet. The ensemble-averaged lambda exonuclease digestion rate of different substrates, including one with an elongated FMR1 gene containing 120 CGG repeats, was measured using absorption and fluorescence spectroscopy. By use of magnetic tweezers sequence-dependent digestion rates and pausing was measured for individual lambda exonucleases. Within the triplet repeats a lower average and narrower distribution of rates and a higher frequency of pausing was observed.
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