期刊
EUKARYOTIC CELL
卷 8, 期 3, 页码 271-277出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/EC.00391-08
关键词
-
资金
- NIH [AI061497, AI06145]
Proper regulation of the cyclic AMP-dependent protein kinase (PKA) pathway is required for normal growth and development in many fungi. We have reported that deletion of the PKA regulatory subunit gene, pkaR, in Aspergillus fumigatus leads to defects in germination and a hypersensitivity of conidia to oxidative stress. In this study, we further analyzed the defects of Delta pkaR conidia and found that a large proportion were abnormally larger than wild type. Because swelling and increased susceptibility to oxidative stress are characteristic of germinating conidia, we analyzed the metabolic activity of the conidia by mitochondrial staining. Whereas it required 4 h in rich medium for wild-type mitochondria to become active, Delta pkaR conidia harbored active mitochondria in the absence of a germinant. Furthermore, conidia of the mutant showed a dramatic loss in viability upon short-term storage in water, indicating starvation-induced death. Taken together, our data suggest that PKA activity regulates metabolic activation of resting conidia. Additionally, the Delta pkaR mutant displayed an abnormal abundance of hyphal nuclei and had increased transcript levels of several cell cycle regulatory genes. These data indicate an important role for PKA in the nuclear duplication cycle of A. fumigatus.
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