期刊
ACS NANO
卷 9, 期 8, 页码 7895-7903出版社
AMER CHEMICAL SOC
DOI: 10.1021/nn5060317
关键词
alpha-hemolysin; nanopore; base identification; truncated pore; toroidal lipid pore
类别
资金
- National Institutes of Health
- Oxford Nanopore Technologies
- BBSRC Doctoral Training Grant
The alpha-hemolysin (alpha HL) protein nanopore has been investigated previously as a base detector for the strand sequencing of DNA and RNA. Recent findings have suggested that shorter pores might provide improved base discrimination. New work has also shown that truncated-barrel mutants (IBM) of alpha HL form functional pores in lipid bilayers. Therefore, we tested IBM pores for the ability to recognize bases in DNA strands immobilized within them. In the case of TBM Delta 6, in which the barrel is shortened by similar to 16 angstrom, one of the three recognition sites found in the wild-type pore, R1, was almost eliminated. With further mutagenesis (Met113 -> Gly), R1 was completely removed, demonstrating that TBM pores can mediate sharpened recognition. Remarkably, a second mutant of TBM Delta 6 (Met113 -> Phe) was able to bind the positively charged beta-cyclodextrin, am(7)beta CD,unusually tightly, permitting the continuous recognition of individual nucleoside monophosphates, which would be required for exonuclease sequencing mediated by nanopore base identification.
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