期刊
EPILEPSY RESEARCH
卷 105, 期 1-2, 页码 220-224出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.eplepsyres.2013.01.003
关键词
SCN1A; CO2; Febrile seizure; Dravet syndrome; GEFS
资金
- Ministry of Education, Culture, Sports, Science, and Technology [21390312, 23659522, 22591130]
- Grants-in-Aid for Scientific Research [21390312, 23659522, 22591130] Funding Source: KAKEN
The aim of this study was to assess the anticonvulsant effect of carbon dioxide (CO2) on Scn1a mutation-related febrile seizures. We examined physiological changes in the blood gas levels after the induction of hyperthermia-induced seizures (HISs), which were associated with the Scn1a missense mutation. We determined the efficacy of inhalation of 5% or 10% CO2 to treat HISs. HISs were evoked in Scn1a mutant and wild-type (WT) rats by hot water baths. To determine the anticonvulsant effect of CO2 inhalation, rats were placed in a chamber filled with air or mixed gas containing 5% CO2 or 10% CO2 for 3 min, immediately after the induction of HISs. We also analyzed the blood gas levels at the end of inhalation of CO2. Hot water bathing induced a significant reduction in the partial pressure of CO2 (pCO(2)) and respiratory alkalosis in the WT and Scn1a mutant rats. HISs were evoked in 100% of the Scn1a mutant rats within 5 min, but in none of the WT rats. The Scn1a mutant rats demonstrated a higher HISs susceptibility associated with respiratory alkalosis than the WT rats. Inhalation of 10% CO2 shortened the seizure duration from 62.6 +/- 12.1 s to 15.5 +/- 1.0 s. Blood gas analysis after the inhalation of 10% CO2 demonstrated an elevated pCO(2) level and respiratory acidosis. Inhalation of 10% CO2 demonstrated a potent and fast-acting anticonvulsant effect against HISs. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据