Surfactant-assisted dispersive liquid-liquid microextraction of nitrazepam and lorazepam from plasma and urine samples followed by high-performance liquid chromatography with UV analysis

Surfactant-assisted liquid-liquid microextraction followed by high-performance liquid chromatography with UV detection has been developed for the simultaneous preconcentration and determination of lorazepam and nitrazepam in biological fluids. In this study, an ionic surfactant (cetyltrimethyl ammonium bromide) was used as an emulsifier. The predominant parameters affecting extraction efficiency such as the type and volume of extraction solvent, the type and concentration of surfactant, sample pH, and the concentration of salt added to the sample were investigated and opted. Under the optimum conditions (extraction solvent and its volume, 1-octanol, 70 mu L; surfactant and its concentration, 1 mL of ultra-pure water containing 2 mmol L-1 cetyltrimethyl ammonium bromide; sample pH = 9 and salt content of 10% NaCl w/v), the preconcentration factors were obtained in the range of 202-241 and 246-265 for nitrazepam and lorazepam, respectively. The limits of quantification for both drugs were 5 mu g L-1 in water sample and 10 mu g L-1 in biological fluids with R-2 values higher than 0.993. The suitability of the proposed method was successfully confirmed by the extraction and determination of the target drugs in human urine and plasma samples in the range of microgram per liter.