期刊
JOURNAL OF SEPARATION SCIENCE
卷 38, 期 10, 页码 1657-1662出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201401387
关键词
Caffeine; Capillary electrophoresis; High-performance liquid chromatography; Ibuprofen; Paracetamol
资金
- CNPq [481683/2013-5, 472465/2012-0]
- FAPEMIG [CEX-APQ-01874-12, CEX-PPM-00503-13]
- Finep
- CAPES
- FAPEMIG
Paracetamol, caffeine and ibuprofen are found in over-the-counter pharmaceutical formulations. In this work, we propose two new methods for simultaneous determination of paracetamol, caffeine and ibuprofen in pharmaceutical formulations. One method is based on high-performance liquid chromatography with diode-array detection and the other on capillary electrophoresis with capacitively coupled contactless conductivity detection. The separation by high-performance liquid chromatography with diode-array detection was achieved on a C-18 column (250x4.6 mm(2), 5 m) with a gradient mobile phase comprising 20-100% acetonitrile in 40 mmol L-1 phosphate buffer pH 7.0. The separation by capillary electrophoresis with capacitively coupled contactless conductivity detection was achieved on a fused-silica capillary (40 cm length, 50 m i.d.) using 10 mmol L-1 3,4-dimethoxycinnamate and 10 mmol L-1 -alanine with pH adjustment to 10.4 with lithium hydroxide as background electrolyte. The determination of all three pharmaceuticals was carried out in 9.6 min by liquid chromatography and in 2.2 min by capillary electrophoresis. Detection limits for caffeine, paracetamol and ibuprofen were 4.4, 0.7, and 3.4 mol L-1 by liquid chromatography and 39, 32, and 49 mol L-1 by capillary electrophoresis, respectively. Recovery values for spiked samples were between 92-107% for both proposed methods.
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