期刊
JOURNAL OF SEPARATION SCIENCE
卷 38, 期 6, 页码 1042-1045出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201400925
关键词
Capillary electrophoresis; On-column complexation; Quantitative metal analysis; Superoxide dismutase
资金
- Estonian Science Foundation [SF0140023s08]
- Strategic Educational Pathways Scholarship, Malta award - European Social Fund Operational Programme II
- University of Malta [PHBRP02-03]
Superoxide dismutases are antioxidant scavenger enzymes that contain a metal cofactor (copper, zinc, iron, and manganese) in their active site. Metal content measurement is one of the essential steps to characterize enzyme biological activity. We have developed a capillary electrophoretic protocol for the determination of the metal content in superoxide dismutase enzymes. The background electrolyte containing 10 mM pyridine-2,6-dicarboxylic acid and 1 mM 1-methyl-3-tetradecylimidazolium chloride at pH 3.8 was optimized for on-column complexation of the above-mentioned metals. The minimum detectable levels of metals ranged from 0.3 to 1.2 mu g/mL. The reliability of the method was checked by parallel quantitative determination of the metal content in superoxide dismutase enzymes by graphite furnace or flame atomic absorption spectrophotometry methods.
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