4.5 Article

Evaluation of new silica-based humic acid stationary phase for the separation of tocopherols in cold-pressed oils by normal-phase high-performance liquid chromatography

期刊

JOURNAL OF SEPARATION SCIENCE
卷 38, 期 5, 页码 813-820

出版社

WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201401377

关键词

Humic acid; Immobilization; Normal-phase high-performance liquid chromatography; Stationary phase; Tocopherol

资金

  1. Selcuk University Coordination of Scientific Research [SU-BAP 14401096]

向作者/读者索取更多资源

A new humic acid stationary phase was prepared by immobilizing humic acid onto aminopropyl silica via an amide linkage formation and used, for the first time, for the separation and quantification of the tocopherol compounds in cold-pressed oil samples under normalphase high-performance liquid chromatography conditions. Parameters affecting the chromatographic separation such as mobile phase composition and flow rate were optimized. By evaluating the calculations of capacity factor, asymmetry factor, resolution, selectivity factor, and theoretical plate number, the best separation was obtained with isocratic elution of n-hexane and isopropyl alcohol (99:1% v/v) at a flow rate of 1.0 mL/min. The effluent was monitored by a fluorescence detector set at excitation and emission wavelengths 295 and 330 nm, respectively. All compounds were separated in 20 min. The method was validated according to international guidelines and found to be linear in a wide concentration range, also the mean recovery of the compounds ranged from 97.9 to 99.2%, with a CV less than 2.7% in all cases. The results showed that the developed stationary phase is suitable for the separation and quantification of the tocopherol compounds in real oil samples.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据