期刊
JOURNAL OF SEPARATION SCIENCE
卷 38, 期 18, 页码 3279-3287出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201500539
关键词
Dummy; Ionic liquids; Molecular imprinting; Molecular simulation; L-phenylalanine
资金
- National Nature Science Foundation of China [21174111, 51433008]
In this study, dummy imprinting technology was employed for the preparation of L-phenylalanine-imprinted microspheres. Ionic liquids were utilized as both a dummy template and functional monomer, and 4-vinylpyridine and ethylene glycol dimethacrylate were used as the assistant monomer and cross-linker, respectively, for preparing a surface-imprinted polymer on poly(divinylbenzene) microspheres. By the results obtained by theoretical investigation, the interaction between the template and monomer complex was improved as compared with that between the template and the traditional L-phenylalanine imprinted polymer. The batch experiments indicated that the imprinting factor reached 2.5. Scatchard analysis demonstrated that the obtained dummy molecularly imprinted microspheres exhibited an affinity of 77.4M.10(-4), significantly higher that of a traditional polymer directly prepared by L-phenylalanine, which is in agreement with theoretical results. Competitive adsorption experiments also showed that the molecularly imprinted polymer with the dummy template effectively isolated L-phenylalanine from L-histidine and L-tryptophan with separation factors of 5.68 and 2.68, respectively. All these results demonstrated that the polymerizable ionic liquid as the dummy template could enhance the affinity and selectivity of molecularly imprinted polymer, thereby promoting the development of imprinting technology for biomolecules.
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