期刊
EPIGENETICS
卷 7, 期 7, 页码 689-694出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/epi.20461
关键词
folic acid; selenium; DNA methylation; CpG island; LINE-1
资金
- BBSRC
- BBSRC's core strategic grant
- BBSRC [BBS/E/F/00044449] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/F/00044449] Funding Source: researchfish
The micronutrients folate and selenium may modulate DNA methylation patterns by affecting intracellular levels of the methyl donor S-adenosylmethionine (SAM) and/or the product of methylation reactions S-adenosylhomocysteine (SAH). WI-38 fibroblasts and FHC colon epithelial cells were cultured in the presence of two forms of folate or four forms of selenium at physiologically-relevant doses, and their effects on LINE-1 methylation, gene-specific CpG island (CGI) methylation and intracellular SAM: SAH were determined. At physiologically-relevant doses the forms of folate or selenium had no effect on LINE-1 or CGI methylation, nor on intracellular SAM: SAH. However the commercial cell culture media used for the selenium studies, containing supra-physiological concentrations of folic acid, induced LINE-1 hypomethylation, CGI hypermethylation and decreased intracellular SAM: SAH in both cell lines. We conclude that the exposure of normal human cells to supra-physiological folic acid concentrations present in commercial cell culture media perturbs the intracellular SAM: SAH ratio and induces aberrant DNA methylation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据