期刊
ACS NANO
卷 9, 期 6, 页码 6354-6362出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b01929
关键词
SERS; immunoassay; ac-EHD; shear force; nonspecific adsorption
类别
资金
- ARC DECRA [DE140101056, DE120102503]
- National Breast Cancer Foundation of Australia [CG-12-07]
- ARC [DP140104006]
- Australian Research Council [DE140101056] Funding Source: Australian Research Council
A rapid and simple approach is presented to address two critical issues of surface-enhanced Raman scattering (SERS)-based immunoassay such as removal/avoiding nonspecific adsorption and reducing assay time. The approach demonstrated involves rationally designed fluorophore-integrated gold/silver nanoshells as SERS nanotags and utilizes alternative current electrohydrodynamic (ac-EHD)-induced nanoscaled surface shear forces to enhance the capture kinetics. The assay performance was validated in comparison with hydrodynamic flow and conventional immunoassay-based devices. These nanoscaled physical forces acting within nanometer distances from the electrode surface enabled rapid (40 min), sensitive (10 fg/mL), and highly specific detection of human epidermal growth factor receptor 2 in breast cancer patient samples. We believe this approach presents potential for the development of rapid and sensitive SERS immunoassays for routine clinical diagnosis.
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