期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 58-59, 期 -, 页码 8-13出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2014.02.004
关键词
Acetylcholinesterase biosensor; Cu-Mg-Al calcined layered double hydroxide; Graphene-gold nanocomposite; Pesticides
资金
- National Natural Science Foundation of China [30972055, 31101286]
- Agricultural Science and Technology Achievements Transformation Fund Projects of the Ministry of Science and Technology of China [2011GB2C60020]
- Shandong Provincial Natural Science Foundation, China [Q2008D03]
In this study, a novel acetylcholinesterase-based biosensor was fabricated. Acetylcholinesterase (AChE) was immobilized onto a glassy carbon electrode (GCE) with the aid of Cu-Mg-Al calcined layered double hydroxide (CLDH). CLDH can provide a bigger effective surface area for AChE loading, which could improve the precision and stability of AChE biosensor. However, the poor electroconductibility of CLDHs could lead to the low sensitivity of AChE biosensor. In order to effectively compensate the disadvantages of CLDHs, graphene-gold nanocomposites were used for improving the electron transfer rate. Thus, the graphene-gold nanocomposite (GN-AuNPs) was firstly modified onto the GCE, and then the prepared CLDH-AChE composite was immobilized onto the modified GCE to construct a sensitive AChE biosensor for pesticides detection. Relevant parameters were studied in detail and optimized, including the pH of the acetylthiocholine chloride (ATCI) solution, the amount of AChE immobilized on the biosensor and the inhibition time governing the analytical performance of the biosensor. The biosensor detected chlorpyrifos at concentrations ranging from 0.05 to 150 mu g/L. The detection limit for chlorpyrifos was 0.05 mu g/L. (C) 2014 Elsevier Inc. All rights reserved.
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