期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 55, 期 -, 页码 1-6出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2013.11.005
关键词
Horseradish peroxidase; Phospholipid-templated titania; Enzyme encapsulation; Phenolic compound; Dye
资金
- National Nature Science Foundation of China [21006020, 21276060, 21276062]
- Application Basic Research Plan Key Basic Research Project of Hebei Province [11965150D]
- Natural Science Foundation of Tianjin [13JCYBJC18500]
In this study, horseradish peroxidase (HRP) was encapsulated in phospholipid-templated titania particles through the biomimetic titanification process and used for the treatment of wastewater polluted with phenolic compounds and dye. The encapsulated HRP exhibited improved thermal stability, a wide range of pH stability and high tolerance against inactivating agents. It was observed an increase in K-m value for the encapsulated HRP (8.21 mM) when compared with its free counterpart. For practical applications in the removal of phenolic compounds and dye by the encapsulated HRP, the removal efficiency for phenol, 2-chlorophenol, Direct Black-38 were 92.99%, 87.97%, and 79.72%, respectively, in the first treatment cycle. Additionally, the encapsulated HRP showed better removal efficiency than free HRP and a moderately good capability of reutilization. (C) 2013 Elsevier Inc. All rights reserved.
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