期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 66, 期 -, 页码 60-66出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2014.08.009
关键词
Graphene; Cobalt phthalocyanine; Glucose oxidase; Biosensor; Amperometry; Practicality
资金
- National Science Council
- Ministry of Education of Taiwan (Republic of China)
We described a simple and facile chemical reduction strategy for the preparation of graphene (GR)-cobalt phthalocyanine (CoPc) composite and explored it for the enzymatic determination of glucose. CoPc is an active mediator and electrocatalysts for the immobilization of GOx and determination of glucose. However, it is not stable on the electrode surface and also suffers from lack of conductivity. Here, we have employed GR as the suitable support to stabilize CoPc through simple chemical reduction method and the resulting composite has been used for the glucose biosensor application. Scanning electron microscopy, X-ray diffraction and Energy-dispersive X-ray spectroscopy studies confirmed the successful formation of composite. Direct electron transfer of glucose oxidase (GOx) was observed with well defined redox peaks a the formal potential of -0.44V: The amount of electroactive GOx (Gamma) and electron transfer rate constant (k(s)) were calculated to be 3.77 x 10(-10) mol cm(-2) and 3.57 s(-1), respectively. The fabricated amperometric biosensor detects glucose in wide linear concentration range from 10 mu M to 14.8 mM with high sensitivity of 5.09 mu A mM(-1) cm(-2). The sensor offered very low detection limit (LOD) of 1.6 mu M. In addition, practical feasibility of the sensor has been explored in screen printing carbon electrode with accurate determination of glucose present in human blood serum and urine samples. Furthermore, the sensor exhibited appreciable stability, repeatability and reproducibility results. (C) 2014 Elsevier Inc. All rights reserved.
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