4.5 Article

Modulation of enzyme catalytic properties and biosensor calibration parameters with chlorides: Studies with glucose oxidase

期刊

ENZYME AND MICROBIAL TECHNOLOGY
卷 53, 期 4, 页码 278-282

出版社

ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2013.02.011

关键词

Modulation; Catalytic activity; Glucose oxidase; Lactose biosensor; Calibration parameter

资金

  1. Estonian Science Foundation [7465, 9061]

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We studied the modulation of calibration parameters of biosensors, in which glucose oxidase was used for bio-recognition, in the presence of different chlorides by following the transient phase dynamics of oxygen concentration with an oxygen optrode. The mechanism of modulation was characterized with the changes of the glucose oxidase catalytic constant and oxygen diffusion constant. The modulation of two biosensor calibration parameters were studied: the maximum calculated signal change was amplified for about 20% in the presence of sodium and magnesium chlorides; the value of the kinetic parameter decreased along with the addition of salts and increased only at sodium chloride concentrations over 0.5 mM. Besides glucose bioassay, the amplification of calibration parameters was also studied in cascaded two-enzyme lactose biosensor, where the initial step of lactose bio-recognition, the beta-galactosidase - catalyzed lactose hydrolysis, was additionally accelerated by magnesium ions. (c) 2013 Elsevier Inc. All rights reserved.

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