期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 51, 期 3, 页码 171-176出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2012.05.010
关键词
Corynebacterium glutamicum; Gamma-amino butyric acid; Glutamate decarboxylase; pyridoxal 5 '-phosphate
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
Gamma-amino butyric acid (GABA) is a component of pharmaceuticals, functional foods, and the biodegradable plastic polyamide 4. Here, we report a simple and robust system to produce GABA from glucose using the recombinant Corynebacterium glutamicum strain GAD, which expresses GadB, a glutamate decarboxylase encoded by the gadB gene of Escherichia coli W3110. As confirmed by HPLC analysis. GABA fermentation by C. glutamicum GAD cultured at 30 degrees C in GABA Production 1 (GP1) medium containing 50 g/L glucose without the addition of glutamate yielded 8.07 +/- 1.53 g/L extracellular GABA after 96h. Addition of 0.1 mM pyridoxal 5'-phosphate (PLP) was found to enhance the production of GABA, whereas Tween 40 was unnecessary for GABA fermentation. Using the optimized GABA Production 2 (GP2) medium, which contained 50 g/L glucose and 0.1 mM PLP, fermentation was performed in a flask at 30 degrees C with 10% (v/v) seed culture of C. glutamicum GAD. GABA was produced in the culture supernatant with a yield of 12.37 +/- 0.88 g/L after 72 h with a space-time yield of 0.172 g/L/h, which is the highest yield obtained to date for GABA from fermentation with glucose as a main carbon source. (C) 2012 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据