期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 49, 期 4, 页码 366-371出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2011.06.013
关键词
Trichoderma reesei; Beta-glucosidase; Cellulase; Heterologous expression; Penicillium decumbens
资金
- Chinese Academy of Sciences [KSCX1-YW-11B3, KSCX2-EW-J-12, GJHZ1128]
- High-tech Research and Development Program of China [2007AA021302]
- National Basic Research Program of China [2011CB707403]
Trichoderma reesei is a well-known cellulase producer and widely applied in enzyme industry. To increase its ability to efficiently decompose cellulose, the beta-glucosidase activity of its enzyme cocktail needs to be enhanced. In this study, a beta-glucosidase I coding sequence from Penicillium decumbens was ligated with the cellobiohydrolase 1 (cbh1) promoter of T. reesei and introduced into the genome of T. reesei strain Rut-C30 by Agrobacterium-mediated transformation. In comparison to that from the parent strain, the beta-glucosidase activity of the enzyme complexes from two selected transformants increased 6- to 8-fold and their filter paper activity (FPAs) was enhanced by 30% on average. The transformant's saccharifying ability towards pretreated cornstalk was also significantly enhanced. To further confirm the effect of heterologous beta-glucosidase on the cellulase activity of T. reesei, the heterologously expressed pBGL1 was purified and added to the enzyme complex produced by T. reesei Rut-C30. Supplementation of the Rut-C30 enzyme complex with pBGL1 brought about 80% increase of glucose yield during the saccharification of pretreated cornstalk. Our results indicated that the heterologous expression of a beta-glucosidase gene in T. reesei might produce balanced cellulase preparation. (C) 2011 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据