期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 46, 期 6, 页码 534-539出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2009.12.017
关键词
White rot fungi; Laccase; Copper induction
资金
- Fundacion Banco Rio and Secretaria de Ciencia y Tecnologia de la Universidad Nacional de Misiones
- CONICET, Argentina
- Comite Ejecutivo de Desarrollo e Innovacion Productiva (CEDIT), Misiones, Argentina
Fungi may be selected as models for gene expression studies and further adaptation for biotechnological enzyme production. The aim of this work was to evaluate laccase production and to analyze the effect of Cu2+ on selected fungi natives of Misiones, Ganoderma applanatum (strain F), Peniophora sp. (BAFC 633), Pycnoporus sanguineus (BAFC 2126) and Coriolus versicolor f. antarcticus (BAFC 266). Fungi secretion system of G. applanatum, Peniophora sp., P. sanguineus and C versicolor f. antarcticus is sensitive to stimulation by copper. Biomass values of G. applanatum, Peniophora sp. and C. versicolor f. antarcticus did not show differences between treatments. P. sanguineus biomass underwent a dramatic growth inhibition with 1 mM Cu2+ and marked delay in growth with 0.5 mM Cu2+. Proteins were increased with copper in Peniophora sp., C. versicolor and G. applanatum. G. applanatum and Peniophora sp. reached the highest enzyme activity at 10th day equivalent to 49.2-fold and 19.7-fold higher than the control samples, respectively. Copper produced an increase of constitutive laccases in all fungi and an additional inducible isoenzyme in Peniophora sp., C. versicolor f. antarcticus and G. applanatum. (C) 2010 Elsevier Inc. All rights reserved.
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