Degradation of wood and enzyme production by Ceriporiopsis subvermispora

The degradation of the components of Japanese beech and Japanese cedar wood was measured overtime in cultures of the white-rot fungus Ceriporiopsis subvermispora. Although there was no initial degradation of cedar wood, after 12 weeks the mass loss of both cedar and beech wood was 15-20%. The mass losses of filter paper in beech wood-containing cultures and glucose cultures after 12 weeks were 87% and 70%, respectively. The ratio of lignin loss to mass loss of both beech and cedar wood cultures approached 2.0. Although the cellulose loss in cedar wood was very low throughout the 12-week incubation, C subvermispora degraded the hemicellulose in Japanese cedar much more effectively than that in Japanese beech. These results confirm that C subvermispora is a selective lignin degrader. During the 12-week incubation with Japanese beech wood, C. subvermispora continuously produced at least one of three phenol oxidases: laccase was produced initially, followed by Mn-independent peroxidase activity peaking at 6 weeks and Mn-dependent peroxidase activity peaking at 10 weeks. Lignin peroxidase and carboxymethylcellulase activities peaked after 3 weeks of incubation. Avicelase activity was present throughout the incubation period, although the activity was very low. The low-molecular-mass fraction of the extracellular medium, which catalyzes a redox reaction between 02 and electron donors to produce hydroxyl radical, may act synergistically with the enzymes to degrade wood cell walls. (c) 2009 Elsevier Inc. All rights reserved.