期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 42, 期 5, 页码 445-449出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2008.01.002
关键词
Penicillium variabile; fermentation; biotransformation; gluconic acid; fed-batch; feeding strategies
Penicillium variabile P16 is characterized by the ability to release high levels of glucose oxidase when grown in glucose-rich media converting, at the same time, glucose into gluconic acid. This work reports on gluconic acid production by the above strain grown in bench-top reactor under different strategies, traditional batch and fed-batch processes; Na(2)CO(3) was substituted for CaCO(3) as the buffering agent in order to prevent calcium gluconate precipitation over the myceliurn that would limit oxygen uptake and, as a consequence, glucose conversion. Best results were obtained with an improved fed-batch process which, in addition to glucose, was continuously fed with nitrogen and minerals at a flow rate of 0.02 ml min(-1): yield and total productivity were 99.4% and 2.02 g h(-1), respectively, and remained constant till the end of the fermentation (240 h). (C) 2008 Elsevier Inc. All rights reserved.
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