期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 42, 期 3, 页码 235-241出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2007.09.014
关键词
alginate gel; biocomposite; double-walled carbon nanotube; lactate dehydrogenase; immobilization
An efficient carrier, double-walled carbon nanotube-doped alginate gel (DWCNT-ALG), was researched for lactate dehydrogenase (LDH, EC 1.1.1.27) immobilization in this study. LDH was pre-adsorbed on DWCNTs and then they were encapsulated in the ALG matrices followed by Ca2+ cross-linking to form LDH-DWCNT-ALG biocomposites. Scanning and transmission electron microscope images were used to characterize the LDH-DWCNT-ALG biocomposites and the DWCNTs before and after being adsorbed by LDH. LDH leakage from LDH-DWCNT-ALG biocomposites was remarkably reduced about 61.7% compared with LDH-ALG biocomposites. The results of the optimum pH and temperature of the reaction catalyzed by immobilized LDH showed that higher activity was obtained by using LDH-DWCNT-ALG biocomposites. Operational and storage stabilities of immobilized LDH had been greatly improved after being immobilized in LDH-DWCNT-ALG hybrid biocomposites. This work showed both the biocompatibility of the organic component and the higher surface energy of nanotubes in enzyme immobilization. (c) 2007 Elsevier Inc. All rights reserved.
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