期刊
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
卷 37, 期 2, 页码 608-616出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.etap.2014.01.017
关键词
Nonylphenol; Cell receptors; Secretory function; TM4 cells
资金
- National Basic Research Program of China (973 program) [2012CB720805]
- International Science & Technology Cooperation Program of China [2010DFA31780]
- Joint Sino-German Research Project of National Natural Science Foundation of China [GZ 731]
- Program for New Century Excellent Talents in University [NCET-12-0749]
The aim of this study was to investigate the effects of nonylphenol (NP) exposure on the expression of cell receptors and secretory function in mouse Sertoli TM4 cells. There were no significant changes in mRNA expression of estrogen receptor (ER)- alpha and toll like receptor (TLR)-4 in the cells exposed. to NP for 24h. However, the mRNA expression levels of ER-beta, progesterone receptor (PR) and androgen receptor (AR) were down-regulated in NP groups. Furthermore, NP treatment evoked significant changes in protein expression levels of ER-beta and follicle-stimulating hormone receptor (FSHR). There were significant changes in the mRNA expression levels of vinculin, N-cadherin and occludin, but not vimentin. Levels of inhibin B, androgen binding protein (ABP) and transferrin (Trf) were found to change significantly in NP challenged cells. Additionally, the decrease of nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) mRNA expression and increase of cytokine levels were simultaneously found in NP stimulated TM4 cells. In conclusion, these findings have shown that NP exposure affected expression of cell receptors and may damage specific secretory function of Sertoli TM4 cells, which may be associated with the male-specific reproductive toxicity of NP. (c) 2014 Elsevier B.V. All rights reserved.
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