期刊
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
卷 37, 期 3, 页码 1048-1059出版社
ELSEVIER
DOI: 10.1016/j.etap.2014.03.020
关键词
Tributyltin chloride; Brain regions; Cell death; Oxidative stress; GFAP; Differential toxicity
资金
- CSIR [SIP-08]
- University Grants Commission (UGC)
Tributyltin (TBT) is a potent biocide and commonly used in various industrial sectors. Humans are mainly exposed through the food chain. We have previously demonstrated tin accumulation in brain following TBT-chloride (TBTC) exposure. In this study, effect of TBTC on dissociated cells from different brain regions was evaluated. Cytotoxicity assay (MTT), mode of cell death (Annexin V/PI assay), oxidative stress parameters (ROS and lipid peroxidation), reducing power of the cell (GSH), mitochondrial membrane potential (MMP) and intracellular Ca2+ were evaluated to ascertain the effect of TBTC. Expression of glial fibrillary acidic protein (GFAP) was measured to understand the effect on astroglial cells. TBTC as low as 30 nM was found to reduce GSH levels, whereas higher doses of 300 and 3000 nM induced ROS generation and marked loss in cell viability mainly through apoptosis. Striatum showed higher susceptibility than other regions, which may have further implications on various neurological aspects. (C) 2014 Elsevier B.V. All rights reserved.
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