期刊
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
卷 36, 期 1, 页码 40-50出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.etap.2013.02.014
关键词
Benzo[a]pyrene; DNA methylation; Zebrafish; Vasa
资金
- National Institute of Environmental Sciences [R01ES012710, R03ES018962, R21ES019940]
- South Central Technology Transfer Award from Society of Toxicology
- Graduate Student Council Research Grant from the University of Mississippi [6402-21310-003-00]
- Agricultural Research Service
DNA methylation is important for gene regulation and is vulnerable to early-life exposure to environmental contaminants. We found that direct waterborne benzo[a]pyrene (BaP) exposure at 24 mu g/L from 2.5 to 96 hpf to zebrafish embryos significantly decreased global cytosine methylation by 44.8% and promoter methylation in uasa by 17%. Consequently, vasa expression was significantly increased by 33%. In contrast, BaP exposure at environmentally relevant concentrations did not change CpG island methylation or gene expression in cancer genes such as ras-association domain family member 1 (rassf1), telomerase reverse transcriptase (tert), c-jun, and c-myca. Similarly, BaP did not change gene expression of DNA methyltransferase 1 (dnmt1) and glycine N-methyltransferase (gnmt). While total DNMT activity was not affected, GNMT enzyme activity was moderately increased. In summary, BaP is an epigenetic modifier for global and gene specific DNA methylation status in zebrafish larvae. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据