Microcystin-LR causes cytotoxicity effects in rat testicular Sertoli cells

Microcystins (MCs) are produced by cyanobacteria. The most toxic and widely distributed MC is microcystin-LR (MC-LR). The aim of this study was to investigate whether exposure to MC-LR could induce oxidative stress, leading the further toxicity effects on Sertoli cells in vitro. Sertoli cells obtained from rats were cultured with a medium containing 0, 0.5, 5, 50 or 500 nM/l MC-LR. We examined the decrease of mitochondrial membrane potential (MMP), the increase of reactive oxygen species (ROS) production, the increase of lipid peroxidation and decrease of superoxide dismutase (SOD) activity in Sertoli cells after treatment with MC-LR in vitro, and higher expression of caspase-9 and caspase-3, the increase of apoptosis rate. Therefore, we deduced that direct exposure to microcystin-LR could induce oxidative stress generation in Sertoli cells, and subsequently depressed cellular viability and caused cells to undergo apoptosis, resulting in the reproductive toxicity in male rats. (C) 2011 Elsevier B.V. All rights reserved.