期刊
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
卷 34, 期 3, 页码 926-934出版社
ELSEVIER
DOI: 10.1016/j.etap.2012.08.003
关键词
Antioxidant; Ecklonia cava; Eckstolonol; Human keratinocytes; Oxidative stress; Ultraviolet-B
资金
- Korea Institute of Ocean Science and Technology [PE 98751, PE 98785, PE 98832]
- Ministry of Land, Transport & Maritime Affairs (MOLIT), South Korea [PE98751] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ultraviolet-B (UV-B) irradiation has been known to generate oxidative stress by increasing reactive oxygen species (ROS) in skin cells. Several naturally occurring antioxidant compounds isolated from marine algae are believed to protect against ROS. In this study, we assessed the antioxidative effect of eckstolonol isolated from Ecklonia cava against UV-B-induced ROS in human keratinocytes (HaCaTs). We investigated the effects of photo-oxidative stress by UV-B (50 mJ/cm(2)) and the antioxidative effects of eckstolonol using fluorometry, flow cytometry, microscopy, and cell viability and comet assays. UV-B irradiation decreased cell viability, which was restored in a dose-dependent manner with eckstolonol treatment (0, 5, 50, 100, and 200 mu M). Moreover, eckstolonol reduced UV-B-induced ROS, lipid peroxidation, damaged DNA levels, and cell death. These antioxidative effects seem to be due to the enzymatic activities of catalase (CAT) and superoxide dismutase (SOD). Collectively, these results indicate that eckstolonol is capable of protecting keratinocytes from photo-oxidative stress. (C) 2012 Elsevier B.V. All rights reserved.
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