期刊
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
卷 34, 期 2, 页码 263-271出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.etap.2012.04.010
关键词
Advanced adipose-derived stem cell protein extracts; Reactive oxygen species; Matrix metalloproteinases; Human dermal fibroblasts
资金
- Ministry of Knowledge Economy of Korea [0801DG10141]
- Ministry of Education through the Brain Korea 21 Project
Advanced adipose-derived stem cell protein extracts (AAPE) were used instead of live stem cells to investigate their effect on oxidative stress and matrix metalloproteinases (MMPs) related to tissue repair in human dermal fibroblasts (HDFs). In this study, it was observed that AAPE at 2 mu g/ml specifically exhibited scavenging activity of hydrogen peroxide and reducing power. The inhibitory effect of AAPE at 2 mu g/ml on MMP-2 activity was increased in the presence of phorbol myristate acetate (PMA). In the absence of PMA, AAPE significantly enhanced activities of MMP-1 and MMP-2 in HDFs, respectively. However, the level of MMP-1 expression was decreased in a dose dependent manner by AAPE. In addition, while the level of extracellular signal-regulated kinases 1 (ERK1) activation was reduced in the presence of AAPE compared to blank, the level that of ERK2 activation was not changed. The expression level of c-Fos, a part of activator protein-1 (AP-1), was increased in nucleus of HDFs. These results reveal that activation of MMPs in the presence of AAPE was increased via AP-1 in HDFs, suggesting that AAPE can be a potential candidate for tissue repair. (C) 2012 Elsevier B.V. All rights reserved.
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