期刊
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
卷 32, 期 3, 页码 364-372出版社
ELSEVIER
DOI: 10.1016/j.etap.2011.08.002
关键词
Breast cancer; Chlorambucil; PAMAM dendrimer; Collagen biosynthesis
资金
- Medical University of Bialystok [3-17554F]
Evaluation of the cytotoxicity of a novel G3 PAMAM-NH2 dendrimer-chlorambucil conjugate employing a MTT assay and inhibition of [H-3]thymidine incorporation into DNA in both MDA-MB-231 and MCF-7 breast cancer cells demonstrated that the conjugate was more potent antiproliferative agent than chlorambucil. It was found that dendrimer-chlorambucil conjugate was more active inhibitor of collagen biosynthesis than chlorambucil. Our experiments carried out with flow cytometry assessment of annexin V binding and fluorescent microscopy assay revealed that PAMAM-CH conjugate inhibited the proliferation of MCF-7 and MDA-231 malignant cells by increasing the number of apoptotic and necrotic cells. The apoptotic effect of PAMAM-CH conjugate was found to be stronger than that caused by chlorambucil. (C) 2011 Elsevier B.V. All rights reserved.
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