期刊
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
卷 29, 期 1, 页码 12-18出版社
ELSEVIER
DOI: 10.1016/j.etap.2009.08.007
关键词
Myricetin; Reactive oxygen species; Antioxidant enzyme; Lipid peroxidation; DNA strand breakage
资金
- Korean Ministry of Knowledge and Economy [70004219]
We evaluated the cytoprotective effect of myricetin on oxidative stress damaged cells by assessment of the scavenging effect of reactive oxygen species(ROS)and the activities of antioxidant enzymes. Myricetin showed the scavenging effect of 1,1 -diphenyl-2-picrylhydrazyl (DPPH) radicals on intracellular ROS. In addition, myricetin restored the activity and protein expression of cellular antioxidant defense enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) reduced by hydrogen peroxide (H2O2) treatment. H2O2-induced cellular DNA and lipid damages, and myricetin was found to prevent the DNA damage shown by inhibition of DNA tail and it decreased nuclear phospho-histone H2A.X expression, which are both markers for DNA strand breakage. Membrane lipid peroxidation was also attenuated as shown by inhibition of TBARS formation and of fluorescence intensity of diphenyl-1-pyrenylphosphine (DPPP). These results suggest that myricetin protects cells against H2O2-induced cell damage via inhibition of ROS generation and activation of antioxidant enzymes. (C) 2009 Elsevier B.V. All rights reserved.
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