期刊
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
卷 26, 期 2, 页码 192-198出版社
ELSEVIER
DOI: 10.1016/j.etap.2008.03.010
关键词
chlorpyrifos; Gambusia affinis; antioxidant enzymes; oxidative stress; acetylcholinesterase; locomotor behaviour
资金
- Council of Scientific and Industrial Research (CSIR)
The recovery effect of chlorpyrifos (CPF) on antioxidant enzymes, locomotor behaviour and the target enzyme acetylcholinesterase (AChE) interaction were studied after exposure to 297 mu g L-1 (LC50 for 96 h) in mosquito fish, Gambusia affinis. Activities of the antioxidant enzymes-superoxide dismutase, catalase, glutathione reductase in viscera, and AChE in brain were inhibited at 96 h of exposure. However, induction in lipid peroxidation was observed. The antioxidant levels were restored to near control by 16-18 days. Similarly, swimming speed and AChE were also recovered but comparatively needs longer period. In vitro AChE study indicated that CPF alters the apparent K. values, resulting in a competitive type of inhibition and the inhibitory constant K-i was found to be 4.57 x 10(-4) M. The results showed that the organophosphate CPF besides its inhibitory effect on target enzyme AChE also inhibits antioxidant enzymes, which can be used as biomarkers in the pesticide-contaminated aquatic streams. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据