MATRIX EFFECT STUDY IN THE DETERMINATION OF LINEAR ALKYLBENZENE SULFONATES IN SEWAGE SLUDGE SAMPLES

We propose a study of the matrix effect in the determination of linear alkylbenzene sulfonates (LAS) in sewage sludge samples. First, a rapid, selective and sensitive method is proposed. The method involves two stages: the extraction of the compound from the samples and analysis by liquid chromatography with fluorescence detection (LC-FLD). Three different techniques of extraction (microwave-assisted extraction, Soxhlet, and ultrasounds) were compared, and microwave-assisted extraction was selected as the best suited for our purpose. Microwave-assisted extraction allows reducing the extraction time (25 min compared with 12 h for conventional Soxhlet extraction) and solvent waste (25 ml of methanol compared with 200 ml for Soxhlet or more than 50 ml for the ultrasonic procedure). Absence of matrix effect was evaluated with two standards (2(sic)C-8:0 and 2(sic)C-16:0) that are not commercial; therefore, neither of them was detected in sewage sludge samples and they showed similar environmental behavior (adsorption and precipitation) to LAS (C-11:0-C-13.0), which allow us to evaluate the matrix effect. Validation was carried out by a recovery assay, and the method was applied to samples from different sources; therefore, they had different compositions. Environ. Toxicol. Chem. 2011;30:813-818. (C) 2011 SETAC