GENOTOXIC EFFECT OF 2,4,6-TRICHLOROPHENOL ON P53 GENE IN ZEBRAFISH LIVER

Trichlorophenol (2,4,6-trichlorophenol [TCP]), a probable human carcinogen, is widely present in the environment. Employing denaturing high-performance liquid chromatography and DNA sequencing analyses, we demonstrated that 10-d exposure of zebrafish to TCP at 5 mu g/L resulted in elevated point mutations of p53 gene in the liver genome. The result suggests that TCP might be involved in carcinogenesis by inducing point mutations in the somatic genome.