期刊
ENVIRONMENTAL TOXICOLOGY
卷 29, 期 8, 页码 893-907出版社
WILEY
DOI: 10.1002/tox.21817
关键词
cadmium; gene expression levels; histology; metallothionein; mitochondria; oxidative stress; crayfish; molecular responses; biomarker; enzymatic activities
资金
- ENVIRHOM research program - Institute of Radioprotection and Nuclear Safety
- CNRS
The present study investigates cadmium effects on the transcription of mitochondrial genes of Procambarus clarkii after acute (0.05, 0.5, and 5 mg Cd/L; 4-10 days) and chronic exposures (10 mu g Cd/L; 30-60 days). Transcriptional responses of cox1, atp6, and 12S using quantitative real-time RT-PCR were assessed in gills and hepatopancreas. Additionally, the expression levels of genes involved in detoxification and/or oxidative stress responses [mt, sod(Mn)] and enzymatic activities of antioxidants (SOD, CAT, GPX, and GST) were analyzed. The histopathological effects in hepatopancreas of crayfish were evaluated by light microscopy. Relationships between endpoints at different levels of biological organization and Cd bioaccumulation were also examined. Cd induced high levels of bioaccumulation, which was followed by mitochondrial dysfunction and histological alterations in both experiments. Moreover, perturbations in the defence mechanisms against oxidative stress tended to increase with time. Results also showed that molecular responses can vary depending on the intensity and duration of the chemical stress applied to the organisms and that the study of mt gene expression levels seemed to be the best tool to assess Cd intoxication. (C) 2012 Wiley Periodicals, Inc.
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