期刊
ENVIRONMENTAL TECHNOLOGY
卷 34, 期 15, 页码 2259-2266出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09593330.2013.765920
关键词
Ulva rigida; Rhizopus oryzae; fish by-products; growth medium; lipase; Staphylococcus xylosus
资金
- Ministry of Higher Education and Scientific Research of Tunisia
Lipase production by Staphylococcus xylosus and Rhizopus oryzae was investigated using a culture medium based on a mixture of synthetic medium and supernatants generated from tuna by-products and Ulva rigida biomass. The proportion of the three medium components was optimized using the simplex-centroid mixture design method (SCMD). Results indicated that the experimental data were in good agreement with predicted values, indicating that SCMD was a reliable method for determining the optimum mixture proportion of the growth medium. Maximal lipase activities of 12.5 and 23.5IU/mL were obtained with a 50:50 (v:v) mixture of synthetic medium and tuna by-product supernatant for Staphylococcus xylosus and Rhizopus oryzae, respectively. The predicted responses from these mixture proportions were also validated experimentally.
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